Tag
His

Part:BBa_K112703:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-10-21)


His affinity tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Construction of His-Tag> basic part K112703

Mix dv009/dv010                        (41bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+41, L)
Product is pBca1256-K112703
-----------------------------------------------
dv009	Forward Biobricking of His-Tag>
cgataGAATTCatgAGATCTatgCATCATCATCATCATCATGGATCCtaacg

dv010   Reverse Biobricking of His-Tag>
cgttaGGATCCATGATGATGATGATGATGcatAGATCTcatGAATTCtatgg

References